Ivana Barbaric

Lecturer in Stem Cell Biology – Department of Biomedical Science

Dr Ivana Barbaric discovered a love of scientific research through competing in a national biology competition she entered in secondary school, for which she won 1st prize. Early on in her career, she realised the importance of networking and began to develop an international network of colleagues, as well as amassing new technical skills, through working and studying abroad. During her PhD in Oxford, she completely changed the direction of her research to focus primarily on a new found fascination with stem cells – a challenging but positive experience. She went on to a postdoc position at the University of Sheffield where she started to think about what she needed to do to obtain an academic position. She made sure to keep publishing in good journals, apply for lots of funding and show her willingness to teach, which she did by taking on undergraduate and Masters student groups and completing a Certificate in Learning and Teaching.

Click here for full staff profile, including research interests, teaching commitments and publication list.

I thoroughly recommend networking… I think it keeps you fresh, you get feedback from your colleagues... and you keep your finger on the pulse of what's really trendy and emerging in your field of research.

Career Timeline

2000

Honours student, Max-Planck Institute for Immunobiology, Freiburg, Germany

2001

BSc with Distinction and First Class Honours in Molecular Biology, University of Zagreb, Croatia

2001–2002

Research Assistant, R&D, Pliva Pharmaceuticals, Zagreb, Croatia

2006

PhD, Department of Pharmacology/MRC Harwell, University of Oxford

2006

Post-doctoral Research Associate, Department of Biomedical Science, University of Sheffield

Second author

Parkinson N, Hardisty-Hughes RE, Tateossian H, Tsai HT, Brooker D, Morse S, Lalane Z, MacKenzie F, Fray M, Glenister P, Woodward AM, Barbaric I, Dear N, Hough TA, Hunter AJ, Cheeseman MT, Brown SD (2006) Mutation at the Evi1 locus in Junbo mice causes susceptibility to otitis media. PLoS Genet 2(10):e149.

2007

Invited talk at Selective Chemical Intervention in Biological Systems, BBSRC Workshop, Manchester

2007

First author

Barbaric I, Wells S, Russ A, Dear TN (2007) Spectrum of ENU-induced mutations in phenotype-driven and gene-driven screens in the mouse. Environ Mol Mutagen 48(2):124-42.

2007

First author

Barbaric I, Stewart M, Wells S, Dear TN (2007) A new coat color mouse line for testing germline transmission of embryonic stem cells while retaining an inbred genetic background. J Am Assoc Lab Anim Sci 46(3):37-40

2007

First author

Barbaric I, Miller G, Dear TN (2007) Appearances can be deceiving: phenotypes of knockout mice. Brief Funct Genomic Proteomic 6(2):91-103

2007

First author

Barbaric I, Dear TN (2007) Optimizing screening and mating strategies for phenotype-driven recessive N-ethyl-N-nitrosourea screens in mice. J Am Assoc Lab Anim Sci 46(6):44-9

2008

Invited talk – 7th Annual International Symposium ‘Human Embryonic Stem Cells’, Sheffield

2008

First author

Barbaric I, Perry MJ, Dear TN, Rodrigues Da Costa A, Salopek D, Marusic A, Hough T, Wells S, Hunter AJ, Cheeseman M, Brown SD (2008) An ENU-induced mutation in the Ankrd11 gene results in an osteopenia-like phenotype in the mouse mutant Yoda. Physiol Genomics 32(3):311-21

November 2008–February 2013

Post-doctoral Research Associate, Department of Biomedical Science, University of Sheffield (on maternity leave from Jan 2012 until Sept 2012)

2009

First author

Barbaric I, Dear T (2009) Culture of murine embryonic stem cells. Methods Mol Biol. 561:161-84

2010

Invited talk – EMBO Course: Anatomy and Embryology of the Mouse, Split, Croatia

2010

Second author

Ashrafian H, Docherty L, Leo V, Towlson C, Neilan N, Steeples V, Lygate CA, Hough T, Townsend S, Williams D, Wells S, Norris D, Glyn-Jones S, Land J, Barbaric I, Lalanne Z, Denny P, Szumska D, Bhattacharya S, Griffin JL, Hargreaves I, Fernandez-Fuentes N, Cheeseman M, Watkins H, Dear TN. (2010) A Mutation in the Mitochondrial Fission Gene Dnm1l Leads to Cardiomyopathy. PLoS Genet 6(6): e1001000

2010

Second author

Harrison NJ, Barbaric I, Andrews PW (2010) Human embryonic stem cell characterization: similarities and differences between cell lines and sources. In: Stem Cells: From Bench to Bedside, 2nd ed. Chapter 2

2010

First author

Barbaric I, Gokhale PJ, Andrews PW (2010) High content screening of small compounds on human embryonic stem cells. Biochem Soc Trans 38(4):1046-50

2010

First author

Barbaric I, Gokhale PJ, Jones M, Glen A, Baker D, Andrews PW (2010) Novel regulators of stem cell fates identified by a multivariate phenotype screen of small compounds on human embryonic stem cell colonies. Stem Cell Res 5(2):104-19

2011

PhD CASE Studentship from AstraZeneca (£30,000) (co-I)

2011

Invited talk – The Krebs Symposium, “-omics revolution”, Sheffield

2011

First author

Barbaric I, Jones M, Harley DJ, Gokhale P, Andrews PW (2011) High-content screening for chemical modulators of embryonal carcinoma cell differentiation and survival. J Biomol Screen 16(6):603-17

2011

First author

Barbaric I, Andrews PW (2011) Drug screens on human stem cells: from understanding cell biology to predicting drug toxicity. Eur Pharm Rev 16(5):52-6

2011

First author

Barbaric I, Jones M, Buchner K, Baker D, Andrews PW, Moore HD (2011) Pinacidil enhances survival of cryopreserved human embryonic stem cells. Cryobiology 63(3):298-305

January 2012–September 2012

Maternity leave

April 2012–present

Visiting Reader, Faculty of Medicine, University of Rijeka, Croatia

2012

Module coordinator for “Stem Cells and Regenerative Medicine”

2012

Invited talk – The Cell Control in a Petri Dish Collaborative Network meeting, London

2012

Second author

Holen I, Whitworth J, Nutter F, Evans A, Brown HK, Lefley DV, Barbaric I, Jones M, Ottewell PD (2012) Loss of plakoglobin promotes decreased cell-cell contact, increased invasion, and breast cancer cell dissemination in vivo. Breast Cancer Res 14(3):R86

2012

First author

Barbaric I, Harrison NJ (2012): Rediscovering pluripotency: from teratocarcinomas to embryonic stem cells. Int J Dev Biol 56(4):197-206

2012

Second author

Lund R, Emani MR, Barbaric I, Kivinen V, Jones M, Baker D, Gokhale P, Nykter M, Lahesmaa R, Andrews PW (2012) Karyotypically Abnormal Human ESCs are Sensitive to HDAC Inhibitors. Stem Cell Research, 11(3):1022-1036

2013

Invited talk – UK-China Stem Cell Initiative Workshop, Shanghai, China

March 2013–February 2014

Wellcome Trust Discipline Hopping Fellow, Department of Materials Science and Tissue Engineering, University of Sheffield

2014

Postgraduate Certificate in Higher Education (PG Cert HE)

March 2014–present

Lecturer, Department of Biomedical Science, University of Sheffield

2014

Organised a workshop: “High Content Screening of Stem Cells: Challenges and Opportunities”, Sheffield

2014

Invited talk – Pluripotent Stem Cell Platform Annual Consortium Meeting, Sheffield

2014

Royal Society Wolfson Grant (£90,000) (co-I)

2014

First author

1. Barbaric I, Biga V, Gokhale PJ, Jones M, Stavish D, Glen G, Coca D, Andrews PW (2014) Time-lapse analysis of human embryonic stem cells reveals multiple bottlenecks restricting colony formation and their relief upon culture adaptation. Stem Cell Reports, 3:142-55

2014–present

Undergraduate & Masters level module teaching (“Tissue Engineering in Biomedical Science” and “Stem Cells”)

2014

Second author

Na J, Baker D, Zhang J, Andrews PW, Barbaric I (2014). Aneuploidy in pluripotent stem cells and implications for cancerous transformation. Protein & Cell, 5:569-79

2015

MRC Confidence in Concept (£49,541)

2015

Invited talk – UK Regenerative Medicine Platform Scientific Showcase, London

2015

Second author

Allison TF, Powles-Glover NS, BigaV, Andrews PW, Barbaric I (2015) Stem cells as tools for high-throughput and high-content screening in drug discovery. Int J High Screen, 5:1-13

2016

MRC Grant (£395,135)

2016

Invited talk – The European Laboratory Research and Innovation Group (ELRIG), Nottingham

2016

Senior author paper

Baker D, Hirst AJ, Gokhale PJ, Juarez MA, Williams S, Wheeler M, Bean K, Allison TF, Moore HD, Andrews PW, Barbaric I (2016) Detecting genetic mosaicism in cultures of human pluripotent stem cells. Stem Cell Reports, 7:998-1012.

2016

Partner in an EU Horizon 2020 project (€650,000)

2016

Inaugural UK Regenerative Medicine Conference, London

2016

Pluripotent learning lounge, Stem Cell Technologies Webinar

2016

International Stem Cell Initiative Workshop: Origins and implications of pluripotent stem cell (epi)genetic instability, Bar Harbor, USA

2016

Stem cell culture conditions and stability workshop, Cambridge

2016

Member of the Steering Committee of the Pluripotent Stem Cell Hub and PluriMes Joint Workshop

2017

Senior author paper

Blair NF, Frith TJR, Barbaric I (2017) Regenerative Medicine: Advances from Developmental to Degenerative Diseases. Personalised Medicine, (pp 225-239).

2017

Other author paper

Scarfe L, Brillant N, Kumar JD, Ali N, Alrumayh A, Amali M, Barbellion S, Jones V, Niemeijer M, Potdevin S, Roussignol G, Vaganov A, Barbaric I, Barrow M, et al (2017) Preclinical imaging methods for assessing the safety and efficacy of regenerative medicine therapies. NPJ Regen Med 19:2-28.

2017

“Genetic Stability in Pluripotent Stem Cells” workshop, Laax, Switzerland

2017

British Society for Gene & Cell Therapy Annual Conference, Cardiff

2017

UK Israel Young Investigator Stem Cell Meeting, Jerusalem, Israel

2017

33rd Annual Meeting European Society of Human Reproduction and Embryology, Geneva

2018

Other author paper

Allison T, Smith AJ, Anastassiasdis K, Sloane-Stanley J, Biga V, Jones M, Coca D, Barbaric I, Gokhale P, Andrews PW (2018) Identification and single cell functional characterisation of a novel endodermally biased pluripotent sub-state in human embryonic stem cells. Stem Cell Reports, 10(6):1895-1907.

2018

Other author paper

Allison T, Andrews P, Avior Y, Barbaric I, Benvenisty N, Bock C, Brehm J, Brüstle O, et al. (2018) Assessment of established techniques to determine developmental and malignant potential of human pluripotent stem cells. Nature Communications, 9(1):1925.

2018

Medical Research Council (MRC): £1,3M

2018

Human Pluripotent Stem Cells and Regenerative Medicine: The Here and Now and Future of our Field, London

2018

Satellite meeting of the TECHNOBEAT Consortium, Weizmann Institute, Tel Aviv, Israel

2018

The Pluripotent and Engineered Stem Cell Platform kick-off meeting, London

2018

Nature Research Round Table: Challenges in ensuring hPSC quality, London

2018

Stem Cell Genomic Integrity conference, the Wellcome Collection, London

2018

Member of the International Stem Cell Initiative Genetics and Epigenetics Study Group

2018

Member of the organising committee of the 3rd Workshop on High Content Screening of Stem Cells, Edinburgh, March 2018

2018

Speaker at the “Soapbox Science” women in STEM event

2019

Senior author paper

Zhang J, Hirst AJ, Duan F, Qiu H, Huang R, Ji Y, Bai L, Zhang F, Robinson D, Jones M, Li L, Wang P, Jiang P, Andrews PW, Barbaric I, Na J. Anti-apoptotic Mutations Desensitize Human Pluripotent Stem Cells to Mitotic Stress and Enable Aneuploid Cell Survival. Stem Cell Reports doi: 10.1016/j.stemcr.2019.01.013.

2019

Laing O, Halliwell J, Barbaric I: Rapid PCR techniques for detecting genetic variants arising in pluripotent stem cells. Current Protocols in Stem Cell Biology (in press).

2019

UKRMP Immunology Kick-off meeting, London

2019

Joint meeting of the Pluripotent Stem and Engineered Cell Hub and the Gene and Cell Therapy Catapult, London

2019

Member of the local organising committee for the Annual Meeting of the British Society of Gene and Cell Therapy

2019

Senior Lecturer in Stem Cell Biology